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高效液相色谱法测定润燥止痒胶囊中苦参碱的含量 被引量:3

Content Determination of Matrine in Runzao Zhiyang Capsule by HPLC
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摘要 [目的]建立高效液相色谱法测定润燥止痒胶囊中苦参碱的含量。[方法]采用Diamonsil钻石C18柱(250.0mm×4.6mm,5μm),以甲醇-0.1%磷酸溶液洗脱(甲醇∶水=50∶50),流速1ml/min,检测波长220nm。[结果]样品中苦参碱平均回收率为98.72%;线性范围:0.2~1.0μg;r=0.9996。[结论]该方法准确,回收率和重现性好,可用于润燥止痒胶囊的质量控制。 [ Objective] The research aimed to establish a new HPLC method for determination matrine in Runzao Zhiyang capsule. [ Method] The content of matrine was determined by using Diamonsil C18 column(250.0 mm ×4.6 mm, 5 μm) ,the mobile phase consisting of methanolwater(0.1% phosphoric acid) elution, with the flow rate at 1 ml/min, the detecting wavelength was 220 nm. [ Result ] The average recovery of matrine in samples were 98, 72%. The linear ranges were 0.2 - 1.0μg , with the regression coefficient of 0. 999 6. [ Conclusion ] The method has satisfactory recoveries and good repeatability. And it can be used in the quality control of Runzao Zhiyang capsule.
出处 《安徽农业科学》 CAS 北大核心 2009年第33期16197-16197,16203,共2页 Journal of Anhui Agricultural Sciences
关键词 高效液相色谱法 润燥止痒胶囊 苦参碱 HPLC Runzao Zhiyang capsule Matrine
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