黑曲霉HQ-1液体发酵产纤维素酶条件研究

Study on the Conditions for Producing Cellulase by Liquid Fermentation with Aspergillus niger HQ-1

[目的]探讨黑曲霉HQ-1液体发酵产纤维素酶的最适条件。[方法]以1株产酶活力相对较高的黑曲霉HQ-1为出发菌株,采用单因子试验和正交试验对黑曲霉HQ-1液体发酵产纤维素酶的最适条件进行初步研究,并测定了CMC酶(CMCase)和滤纸酶(FPAase)的活力。[结果]影响黑曲霉HQ-1液体发酵产CMCase和FPAase的因素依次是复合碳源的含量、2种碳源的比例、氮源含量和装液量。该菌产CMCase的最适培养基为:麸皮+玉米秸秆粉15‰(1∶1),NH4Cl2.0‰,其他成分同Mandel’s营养液;产FPAase的最适培养基为:麸皮+玉米秸秆粉20‰(1∶1),NH4Cl1.5‰,其他成分同Mandel’s营养液。2种培养基的最适pH值和装液量均分别为5.0和50ml/250ml三角瓶。在30℃和170r/min下培养4d后,CMCase和FPAase达到525.2和217.6IU。[结论]该研究为纤维素酶生产菌株的研发提供一定的技术参考。

[ Objective ] The study aimed to discuss the optimum condition for producing cellulase by liquid fermentation of AspergiUus niger HQ-1. [ Method] With 1 strain of A. niger HQ-1 with higher activity of producing cellulase as the original srtain, the optimum conditions for liquid fermentation of A. niger HQ-1 to produce cellulase was studied primarily through the single factor experiments and orthogonal tests and the activities of CMCase and FPAase were measured. [ Result] The factors influencing the production of CMCase and FPAase by liquid fermentation of A, niger HQ-1 were in order were the content of compound carbon origin, the ratio of 2 kinds of carbon, the content of nitrogen and the hquid volume in flask. The optimal medium of producing CMCase was as follows： 15‰ bran ＋ corn stalk powder （ 1 ： 1 ）, 2.0‰ NH4Cl, other components as same as Mandel＇ s medium. The optimal medium of producing FPAase was as follows： 20‰ bran ＋com stalk powder （1： 1）, 1.5%o NH4Cl, others were as same as Mandel＇ s medium. For both medium, the optimum pH was 5.0 and medium volume was 50 ml/250 ml triangle flask. The activities of CMCase and FPAase reached 525.2 and 217.6 IU respectively after culturing for 4 d under 30 ℃ and 170 r/min. [ Conclusion] This study provided some technique reference for the research and development of producing cellulase strain.