延边黄牛GHR基因第8外显子一个新多态位点的发现

Discovery on a New Polymorphic Site of GHR Gene Exon 8 in Yanbian Cattle

[目的]寻找可用于标记辅助选择的分子标记,为延边黄牛分子生物学选种提供依据。[方法]以46头纯种延边黄牛血样为试材,,利用PCR-SSCP技术对GHR基因的第8外显子进行检测并测序,检测了延边黄牛群体内GHR的遗传变异。[结果]对扩增产物进行的SSCP检测发现,有3种基因型,分别为AA、BB、AB,其中AA、BB为纯合子,AB为杂合子。以AA和BB个体为样本的测序分析发现,有一个新多态性位点。GHR基因多态位点遗传分析表明,AA型个体较多,AB型和BB型个体较少。GHR基因的AA基因型效应在部分生长性状上优于其他基因型,差异达到显著水平。[结论]研究提示,GHR基因有可能作为延边黄牛生长性状的候选基因;在育种时应结合多个基因位点才能达到有效选育目的。

[ Objective] The study aimed to seek for molecular marker used in the marker aid selection and provide the basis for molecular breeding of Yanbian cattle. [ Method ] With the blood samples from 46 pure breeds of Yanbian cattle as tested materials, the exon 8 of GHR gene was detected and sequenced by using PCR-SSCP technique and the genetic variation of GHR in Yanbian cattle population was determined. [ Result] SSCP detection on amplified product discovered tha there were 3 genotypes as AA, BB and AB, in which, AA and BB were homozygous and AB was heterozygous. The sequencing analysis with AA and BB as the individuals discovered that there was a new polymorphic site. The genetic analysis on the polymorphic site of GHR gene showed that AA individuals were more and AB and BB individuals were less. AA genotype effect was superior to that of other genotypes on some growth traits, with the difference being significant among genotypes. [ Conclusion] This study suggested that GHR gene may be a candidate gene responsible for growth trait in Yanbian cattle and more gene sites should be combined in breeding so as to realize the effective breeding.