均匀设计法优化短果杜鹃高效快繁体系

Optimizing High Efficient Micropropagation System of Rhododendron brachycarpum D. Don by Using Uniform Design Method

[目的]建立短果杜鹃高效快繁体系,实现短果杜鹃的高效离体快繁。[方法]以短果杜鹃嫩茎段为外植体,应用均匀设计法筛选最适合的培养基。[结果]最适合嫩茎段的腋芽萌发及生根的最佳培养基为MS(改良)+IAA0.15mg/L+IBA0.30mg/L+GA33.00mg/L,再生率达92%以上。以再生植株的茎节为材料进行快繁的结果表明,在35d的1个培养周期内增殖倍数平均达45以上。[结论]该研究建立了短果杜鹃的高效快繁体系,为长白山高山杜鹃的开发利用和工厂化育苗提供了依据。

[ Objective] To establish high efficient micropropagation system of Rhododendron brachycarpum D. Don, and realize the high efficient in vitro micropropagation of R. brachycarpum. [ Method] Young stems of R. brachycarpum were used as explants, suitable medium compositions for axillary bud growing and rooting were screened through uniform design experiments. [ Result] MS （modified） ＋ IAA 0.15 mg/L ＋ IBA 0.30 mg/L ＋ GA3 3.00 mg/L was the most suitable medium with the regeneration rate of 92%. Stems each with one node were cut from regenerated shoots and cultured for propagation, the proliferative multiple was over 45 within one culture period of 35 days. [ Conclusion] High efficient micropropagation system of R. brachycarpum has been successfully established, which provides some basis for development and utilization and industrial seedling of the alpine rhododendron in Changbaishan.