碳离子辐照对甜高粱种子萌发及幼苗酶活的影响

Effect of ^(12)C^(+6) Ions Beam Irradiation to Sorghum bicolor L. on Seed Germination and Enzymes Activity in Seedlings

[目的]研究辐射能量为100MeV/u时不同剂量的碳离子辐照对2个甜高粱品种的种子萌发及几种酶活性的影响,为甜高粱品种的选育提供理论依据。[方法]甜高粱种子萌发后,统计发芽势和成苗率,并测定幼苗叶片中几种酶的活性。[结果]随着剂量的增加,发芽势先升后降,而成苗率逐渐下降,呈"类肩形";GSH-Px、CAT、SOD及LDH酶活性的总体趋势是随着剂量的增加先升后降,不同酶略有不同。[结论]低剂量辐射促进了种子萌发,使酶的活性维持在较高的水平;高剂量时酶活下降,会抑制甜高粱的生长。

[ Objective] The aim was to study the effect of ^12C ^＋6 ions beam irradiation to two varieties of Sorghum bicolor L. on seed germination and some enzymes activity in seedlings with different doses, and provided a theoretical foundation for breeding Sorghum bicolor. [ Method] After germination, germination potential, germination fraction and enzyme activity were detected respectively. [ Result ] The results showed that with the dose increased, the germination potential of Sorghum bicolor increaseed first and then decreases, while their germination fraction presented ＂shoulder like shape＂ ; the activity of LDH, SOD, CAT and GSH-Px increased first and then decreases with doses, they presented slight differences among different enzymes. [ Conclusion] Low dose radiation could accelerate germination of sweet sorghum seeds and enzyme activity could remain at a relatively high level. Enzyme activity decreased with high doses and the growth of Sorghum bicolor was inhibited.