摘要
[目的]构建我国芒果主要栽培品种的AFLP分子标记体系。[方法]以4个芒果品种为材料探索提取高质量DNA的方法,并利用AFLP分子标记在DNA水平上对芒果进行遗传多样性研究。[结果]从64对选择性扩增引物中筛选获得14对多态性强、带型好、分辨率高的组合。应用所筛选的引物对芒果31个主要栽培品种进行指纹图谱分析,结果显示14对引物组合对31个品种扩增出的多态性比率达到97%。[结论]利用AFLP可以高效检测芒果种质资源分子标记多样性。
[ Objective ] The study aimed to construct the AFLP technological system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 Mangifera indica resources was carried out to explore the variety of genetic diversity. [ Result] 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analysis the fingerprint of 31 Mangifera indica cultivars, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs df primers reached to 97% in 31 Mangifera germplasm resources. [ Conclution] It was suggested that AFLP was fit for determing the polymorphism of Mangifera indica resources.
出处
《安徽农业科学》
CAS
北大核心
2009年第33期16305-16307,共3页
Journal of Anhui Agricultural Sciences
基金
国家科技基础平台重点项目(2005DKA21005)
国家自然科学基金项目(30260068)
关键词
芒果
种质资源
AFLP
遗传多样性
Mangifera indica
Germplasm resourse
AFLP
Genetic diversity
