电位控制血红蛋白(Hb)组装制备无媒介体过氧化氢生物传感器

Preparation of Hydrogen Peroxide Biosensor without Intermedium by Electric Potential Controlling of Hb

通过电位控制的方法将血红蛋白Hb固定在由L-半胱氨酸自组装修饰的金电极上制得过氧化氢传感器Hb/L-cys/Au,并通过电化学方法(CV)、原子力显微镜(AFM)等手段对电极的修饰过程进行了表征。试验显示,该传感器对H2O2催化还原性能优良、灵敏度高、稳定性好且非常易于制做。电极在(pH值5.5)0.1mol/LPBS中对H2O2催化还原响应良好,线性可分为2段,低浓度段为8.21×10-8～4.83×10-6mol/L,高浓度段为4.83×10-6～8.22×10-3mol/L,检测限为8.24×10-9mol/L。

Hemoglobin was immobilized on L-cysteine modified gold electrode by using the method of electric potential controlling to develop a new hydrogen peroxide biosensor Hb/L-cys/Au. The modified process of electrode was characterized by the means of CV and AFM. The results showed that the sensor had excellent catalysed reduction performance to H2O2, high sensitivity and good stability. And it was easy to be produced. This electrode presented an excellent electrochemical response for the determination of H2O2 concentration with high sensitivity in 0.1 mol/L PBS （pH value of 5.5）. The linear scope could be divided into 2 sections of concentration section （8.21×10^-8 -4.83 × 10^-6 mol/L） and low concentration section （4.83 × 10 ^-6 - 8.22 × 10^ -3 mol/L）. The detection limit was 8.24 ×10^ -9 mol/L.