摘要
目的：研究乙型肝炎病人的YMDD变并情况。方法：采用双探针实时荧光定量聚合酶链反应（FQ—PCR）对46例HBV—DNA阳性的标本进行YMDD变异株的检测，其中未进行抗病毒治疗的20例样本，进行抗病毒治疗26例。结果：在26例抗病毒治疗的标本中检测出YMDD16例，在未经抗病毒治疗20例标本中检测出YMDD2例。这两组患者之间YMDD变异有显著性差异（P〈0．05）。结论：在未经任何药物治疗的乙型肝炎患者中已经存在YMDD变异株，而在使用拉米夫定治疗的患者中变异株明显增加，因此对乙肝患者在用药前及用药过程中进行YMDD变异株的检测是非常必要的。
Objective ： Research YMDD mutation in hepatitis B patients. Methods ： Double - probe real - time fluorescence quantitative polymerase chain reaction （ FQ - PCR） method was used to detect YMDD mutant in 46 cases of HBV - DNA - positive specimens, Which contains 20 cases of no anti - viral treat- ment and 26 cases of anti - viral treatment. Results ： 16 cases of YMDD were detected from the 26 specimens with anti - virus treatment , while 2 cases of YMDD were detected from the 20 specimens with no anti - virus treatment. There was significant difference （ P 〈 0.05 ） . Conclusion ： YMDD mutation exists in hepatitis B patients who are treated without any drug . However, YMDD mutation increased romarkably among those Hepatitis B patients with the use of lamivudine treatment. So that detection of YMDD mutant is very necessary for those patients before and in the process of drug - using.
