摘要
目的初探山慈姑及其伪品对酪氨酸酶活性的影响。方法采用多巴色素法,底物为L-多巴,测定山慈姑及其伪品提取物对酪氨酸酶的激活作用;薄层色谱法对药材中的酪氨酸进行定性鉴别。结果山慈姑的激活作用最高,丽江山慈姑和金果榄作用不明显,白及呈抑制作用;薄层检测显示山慈姑、丽江山慈姑和金果榄中含有酪氨酸。结论初步认为酪氨酸为山慈姑等激活酪氨酸酶活性作用的主要成分之一。
Objective The effects of Cremastra appendiculata(D.Don) Makino and spurious breed on the activity of tyrosinase were studied.Methods Tyrosinase activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reactiom mixture was measured by spectrophotometer.Tyrosine was identified by TLC.Results The results showed that the Cremastra appendiculata(D.Don) Makino significantly increased the activity of tyrosinase,but the Rhizoma Bletillae inhibited . The Cremt^tra appendiculata (D. Don) Makino, Iphigenia indica Kunth et Benth and Tinospora capilipes Gagnep had tyrosine. Conclusion Tyrosine is one of the major components of the Cremastra appendiculata ( D. Don) Makino, Iphigenia indica Kunth et Benth and Tinospora capilipes Gagnep to stimulate the activity of tyrosinase.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第11期2769-2770,共2页
Lishizhen Medicine and Materia Medica Research
基金
贵州省中药现代化课题(No.[2007]5021)
北京大学天然药物及仿生药物国家重点实验室开放基金
关键词
山慈姑
伪品
酪氨酸酶
酪氨酸
激活作用
Cremastra appendiculata Makino
Spurious breed
Tyrosinase
Tyrosine
Effect