初探山慈姑及其伪品对酪氨酸酶活性的影响

Effects of Cremastra appendiculata(D.Don) Makino and Spurious Breed on Activity of Tyrosinase

目的初探山慈姑及其伪品对酪氨酸酶活性的影响。方法采用多巴色素法,底物为L-多巴,测定山慈姑及其伪品提取物对酪氨酸酶的激活作用;薄层色谱法对药材中的酪氨酸进行定性鉴别。结果山慈姑的激活作用最高,丽江山慈姑和金果榄作用不明显,白及呈抑制作用;薄层检测显示山慈姑、丽江山慈姑和金果榄中含有酪氨酸。结论初步认为酪氨酸为山慈姑等激活酪氨酸酶活性作用的主要成分之一。

Objective The effects of Cremastra appendiculata（D.Don） Makino and spurious breed on the activity of tyrosinase were studied.Methods Tyrosinase activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reactiom mixture was measured by spectrophotometer.Tyrosine was identified by TLC.Results The results showed that the Cremastra appendiculata（D.Don） Makino significantly increased the activity of tyrosinase,but the Rhizoma Bletillae inhibited . The Cremt^tra appendiculata （D. Don） Makino, Iphigenia indica Kunth et Benth and Tinospora capilipes Gagnep had tyrosine. Conclusion Tyrosine is one of the major components of the Cremastra appendiculata （ D. Don） Makino, Iphigenia indica Kunth et Benth and Tinospora capilipes Gagnep to stimulate the activity of tyrosinase.