大豆苷元对离体心脏缺血再灌注损伤的保护作用

The Therapeutic Effects of DD on Isolated Heart Ischemia/Reperfusion Injury

目的研究大豆苷元(DD)对离体心脏缺血再灌注损伤的保护作用。方法应用大鼠离体心肌缺血再灌注损伤模型,在灌流液中加入高、低剂量的大豆苷元。测定冠脉流量、心率、左心室收缩压、左心室内压最大变化速率及心肌组织乳酸脱氢酶(LDH)和心肌肌酸激酶(CK)活性的变化。结果与溶剂组比较,大豆苷元能使左心室收缩压升高、左心室内压最大变化速率增大;能使心肌LDH及CK的活性升高;冠脉流量、心率未见明显变化。结论大豆苷元可通过增大左心室内压最大变化速率、增强心肌收缩力及减少心肌酶的释放对离体心脏缺血再灌注损伤起保护作用。

Objective To explore the therapeutic effects of daidzein（DD） on isolated myocardial ischemia/reperfusion injury.Methods A rat myocardial ischemia/reperfusion injury model was made and was treated with DD（high dose and low dose）.The changes of coronary artery blood flow levels,heart rate,and left ventricular systolic pressure,as well as the activities of lactate dehydrogenase（LDH） and creatine kinase（CK） were investigated.Results Compared with the DMSO group,after treatment, the isolated myocardial tissue showed that the left ventricular systolic pressure, the left ventricular diastolic pressure＇s changing speed increased, as well as enzymatic activities of LDH and CK in myocardial tissue increased. The coronary artery blood flow levels and the heart rates showed no obvious change. Conclusion DD has therapeutic effect on myocardial ischemia/reperfusion injury because it dilates the enhances myocardial contractile force , strongthen the the left ventrieular diastolic pressure＇s changing speed and limits the release of CK and LDH.