温莪术内生真菌的分离及其分类鉴定

Isolation and Identification of Endophytic Fungi from Curcuma aromatica Salisb

目的对温莪术不同部位内生真菌进分离和分类鉴定。方法采用内生菌常规分离法对健康温莪术根、茎和叶的内生真菌进行了分离和鉴定。结果从温莪术中分离得到了99株内生真菌,经鉴定为21属,其中根部42株涉及11属,茎部12株涉及4属,叶部45株涉及12属。结论温莪术的不同部位内生真菌的数量、分布和种群存在差异。

Objective To isolate and identify the endophytic fungi of different positions in Curcuma aromatica Salisb.Methods Endophytic fungi were isolated from healthy roots,stems and leaves of Curcuma aromatical Salisb and identified with conventional method.Results Ninty-nine strains of endophytic fungi belong to 21 genus were isolated and morhpologically identified from Curcuma aromatica Salisb.Forty-two strains of 11 genera were isolated in roots,12strains of 4 genera in stems,and 45 strains of 12 genera in leaves. Conclusion The quantity,population and distribution of the endophytic fungi are various in different position of Curcuma arornatica Salisb.