当归多糖对小鼠肝细胞辐照后Bcl-2和Bax蛋白表达的影响

Effect of polysaccharides from Angelica sinensis on Bcl-2 and Bax protein expression of irradiated liver cells

目的探讨当归多糖ASP3对小鼠肝细胞培养液辐照后凋亡相关基因bcl-2和bax的蛋白表达的影响。方法以当归多糖的自制提取物ASP3为受试物，用2．0Gy ^60Coγ射线照射小鼠肝细胞。采用免疫组织化学方法，检测辐射损伤诱发的肝细胞凋亡相关基因bcl-2和bax的蛋白表达。结果辐射对照组的Bcl-2蛋白表达阳性减弱（55．60％），Bax表达明显增多（70．83％），与正常对照组比较，差异有统计学意义（P〈0．01）。当归多糖ASP3能够调节辐照肝细胞Bcl-2家族蛋白的表达，即抑制Bax的高表达（64．14％／58．37％），同时提高Bcl-2的表达量（59．21％／67．45％），且高剂量（100mg／L）ASP3组与辐射对照组比较，差异有统计学意义（P〈0．05）。结论当归多糖ASP3对辐射诱导的肝细胞凋亡有抑制作用，并通过提高Bcl-2／Bax比值减少细胞凋亡的发生，进而提高肝细胞的DNA损伤修复能力和辐射耐受性。

Objective To investigate the effect of polysaeehafides from Angelica sinensis （ASP3） on Bcl-2 and Bax protein expression of irradiated liver cells from mice. Methods Bel-2 and Bax protein expression of liver cells in vitro exposed to 2.0 Gy rays were examined by using immunohistoehemistry method. Results The expression of apoptosis-aeeelerating protein Bax in the irradiation group was enhanced obviously（70.83 % ）, while apoptosis inhibiting protein Bel-2 tended to decline （55.60 % ）, with the statistically significant difference （ P 〈 0.01 ） compared with that of the control. ASP3 pretreatment could regulate Bcl-2 and Bax protein expression of liver cells, inhibiting Bax protein expression（64.14/58.37 % ） and increasing Bcl-2 protein expresslon（59.21% / 67.45%）. The differences between the high dosage （100 mg/L of ASP3） and the irradiation group were statistically significant（ P 〈0. 05 ）. Conclusions ASP3 pretreatment could prohibit the apoptosis of radiation- damaged liver cells due to abnormal expression of Bel-2 and Bax, and reduce the cell apoptosis by increasing Bcl-2/Bax protein expression so as to enhance the radiation endurance of liver cells.