光化学疗法对内皮细胞管腔形成能力及整合素表达的影响

Effects of photochemotherapy on angiogenic ability of endothelial cells and expression of integrin

目的探讨光化学疗法对内皮细胞管腔形成能力及整合素表达的影响。方法应用体外管腔形成试验(UVA照射剂量分别为0、2.0、5.0 J/cm2)观察光化学疗法对内皮细胞管腔形成能力的影响,同时应用逆转录链式酶反应及流式细胞术分别检测光化学疗法对内皮细胞整合素mRNA及蛋白表达的影响。试验中根据诱导剂分为bFGF刺激组和PMA刺激组,每组内又根据UVA的剂量(0、2.0、5.0 J/cm2)分为三个亚组。结果2.0和5.0 J/cm2的长波紫外线联合100 ng/mL的8-MOP可在体外明显抑制内皮细胞的管腔形成能力,并下调整合素mRNA及蛋白的表达。结论光化学疗法可能通过下调整合素的表达抑制内皮细胞管腔形成能力的抑制效应。

Objective To investigate the effects of photochemotherapy on the angiogenic ability of endothelial cells and expression of integrin. Methods In vitro angiogenesis assay（UVA exposure dose： 0,2.0,5.0 J/cm^2） was used to detect the effects of photochemotherapy on the angiogenic ability of endothelial cells.Reverse polymerase chain reaction and flow cytometry were employed to determine the effects of photochemotherapy on the expression of integrin mRNA and protein,respectively.bFGF stimulation group and PMA stimulation group were divided according to the inductors,and subgroups were divided according to the UVA exposure doses of 0,2.0 and 5.0 J/cm^2 in each group. Results Combination of UVA（2.0 and 5.0 J/cm^2） and 8-MOP（100 ng/mL） resulted in a decrease in the angiogenic ability of endothelial cells in vitro and the expression of integrin mRNA and protein. Conclusion Photochemotherapy may inhibit the angiogenic ability of endothelial cells through downregulating the expression of integrin.