血清HBeAg阴性慢乙肝患者血清IL-27的检测及其意义

The assay and significance of IL-27 in serum of HBeAg negative chronic B-type hepatitis

目的探讨HBeAg阴性慢乙肝患者血清IL-27的变化规律及其临床意义。方法采用酶联免疫吸附法(ELISA)检测血清IL-27的浓度;荧光定量聚合酶链反应法(FQ-PCR)检测HBVDNA;全自动生化仪检测肝功能。结果HBeAg阳性慢乙肝组(n=38)、HBeAg阴性慢乙肝组(n=56),血清IL-27浓度分别为(22.697±20.767)pg/ml、(34.631±22.154)pg/ml。HBeAg阴性慢乙肝组血清IL-27浓度显著高于HBeAg阳性慢乙肝组(P<0.05)。结论IL-27在慢性乙型肝炎感染机制中,可能存在一定的抗病毒作用,可能在促进HBeAg转阴以及抗-HBe的产生过程中发挥了积极作用。

Objective To investigate the change and clinical significance of serous chemokine IL - 27 in patients of HBeAg negative chronic B - type hepatitis. Methods The serous concentration of IL - 27 was detected by enzyme linked immunosorbent assay（ELISA） ; HBV -DNA was detected by fluorescent quantitive polymerase chain reaction（ FQ -PCR） ; Liver function was assayed by automatic biochemistry analyzer. Results Concentration of serous IL - 27 in HBeAg positive chronic B - type hepatitis group （ n = 38 ）, HBeAg negative chronic B - type hepatitis group （ n = 56 ）, was （ 22. 697 ± 20. 767 ） pg/ml, （34. 631 ± 22. 154） pg/ml respectively. The concentration of IL - 27 in HBeAg negative chronic B - type hepatitis group was significantly higher than that of HBeAg positive chronic B - type hepatitis group, （P 〈 0. 05 ）. Conclusion It is possible that IL - 27 promote immune clearance in the pathogenesy of chronic hepatitis B. It may affect actively on the processes of the disappearance of HBeAg and appearance of HBeAb. However, in chronic hepatitis B it may have some cytopathic effect（CPE）.