腹腔冲洗液检测CEA预测胃癌腹膜种植转移的临床意义

The clinical significance of detecting CEA of peritoneal washing in predicting peritoneal metastasis of gastric carcinoma

目的探讨检测胃癌腹膜转移诊断的方法。方法收集50例胃癌患者(胃癌组)及10例胃良性病变患者的腹腔冲洗液,并以胃癌原发灶组织标本作为对照。采用流式细胞术检测标本中肿瘤标志物CEA,采用HE染色进行腹腔冲洗液细胞学(PLC)检查,同时分析上述资料的临床意义。结果胃癌组腹腔冲洗液中CEA阳性表达率为54.0%(27例),明显高于PLC所检测的24.0%(12例)的阳性率(P<0.05)。阳性检出率随着肿瘤浸润深度,TNM分期,胃壁受侵程度的增加而增加。胃癌组原发灶中CEA的阳性表达率为86.0%(43例),对照组腹腔冲洗液中CEA无阳性表达。结论流式细胞术检测腹腔冲洗液中CEA可作为预测胃癌腹膜种植转移的手段。

Objective To explore a method for predicting peritoneal metastasis in gastric carcinoma patients. Methods Peritoneal washings and clinical data of 50 patients with gastric carcinoma and 10 patients with benign gastric diseases were collected. The CEA of peritoneal washings was detected by flow cytometry, the peritoneal lavage cytology （PLC） was examined by HE stain, and the clinical significance of the above-mentioned data was analyzed. Results The positive rate of CEA in peritoneal washings of gastric carcinoma group was 54.0% （27 cases ） , the positive rate of CEA was significantly higher than the PLC detection rate （ 24 % , 12 cases ）. The positive rate of CEA increased with the depth of tumor invasion, TNM staging and degree of peritoneum involvement increased. Conclusions Detection of CEA and other markers in peritoneal washings by flow cytometry can be used to predict peritoneal metastasis of gastric carcinoma.