渥曼青霉素对人胃癌细胞的作用及机制

The effect and mechanism of wortmannin on human gastric carcinoma cells

目的探讨渥曼青霉素(wortmannin)对人胃癌细胞的作用及机制。方法采用10,30,60nmol/L的AKT抑制剂wortmannin,以不同时间对胃癌细胞SGC7901进行干预;并设对照组运用MTT法检测细胞的增殖抑制情况;用Westernblot检测NF-κB核蛋白表达水平;RT-PCR检测NF-κB基因的转录水平。结果3种浓度的wortmannin都对胃癌SGC7901细胞的生长均具有抑制作用,其抑制效应呈明显剂量和时间依赖性(P<0.01或P<0.05);而对照组未见明显变化(P>0.05)。随着wortmannin作用时间的延长,NF-κBmRNA及核蛋白的表达水平均显著低于对照组(P<0.05)。结论wort-mannin可抑制胃癌SGC7901细胞的生长;可能通过胃癌中存在的AKT/NF-κB信号通路调节NF-κB的表达而发挥作用。

Objective To explore the effect and mechanism of wortmannin on the human gastric carcinoma cells. Methods SGC7901 cells were treated with 10nmol/L,30nmol/L and 60nmol/L of wortmannin, a specific inhibitor of AKT, for different time periods. Cell viability was estimated by MTT assay. Western blot was used to detect the level of NF-κB neucleoprotein and reverse transcriptasepolymerase chain reaction （ RT-PCR ） was used to determine transcription of NF-κB mRNA. Results All three different concentrations of wortmannin could inhibit the growth of SGC7901 ceils, and the depression effect obviously depended on time and drug dose（ P 〈 0.01 and P 〈 0.05 ）. However, the cell viability was no change in the control group （ P 〉 0. 05 ）. As the action time of wortmannin was prolonged, neucleoprotein and mRNA expression of NF-κB significantly decreased compared with the control （ P 〈 0.05 ）. Conclusions The findings suggest that wortmannin can inhibit the growth of gastric carcinoma SGC7901 cells, and the action may depend on an AKT/NF-κB pathway in gastric carcinoma cells.