体外培养人牙周牙髓相关细胞中釉原蛋白的表达

Amelogenin expression in cultured human dental pulp and periodontium related cells

目的:观察釉原蛋白(amelogenin,Am)基因在体外培养的人牙龈上皮细胞及口腔外胚间充质来源细胞(人牙龈成纤维细胞、人牙周膜成纤维细胞和人牙髓细胞)中的表达。方法:采用反转录多聚酶链式反应(RT-PCR)技术,检测培养细胞中釉原蛋白mRNA的表达,采用蛋白质免疫印迹技术检测培养细胞中釉原蛋白的表达。结果:培养的人牙周膜成纤维细胞、牙髓细胞、牙龈成纤维细胞和牙龈上皮细胞中均未检测到釉原蛋白及其mRNA的表达。结论:体外培养的人牙周膜成纤维细胞、牙髓细胞、牙龈成纤维细胞和牙龈上皮细胞不表达釉原蛋白基因。

PURPOSE： To investigate gene expression of amelogenin （Am） in human gingival epithelial eellS（HGEC） and also other oral ectomesenchyme cells （human gingival fibroblasts, human periodontal ligament fibroblasts and human pulp cells）. METHODS： The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction （RT-PCR）,and the protein expression was studied with Western blotting. RESULTS： There was no amelogenin expression detected in any of the cells. CONCLUSIONS： These findings suggest that amelogenin expression could not be detected in cultured human periodontium-related cells. Supported by National Natural Science Foundation of China （Grant No.30672315） and Research Fund of Science and Technology Commission of Shanghai Municipality（Grant No.08DZ2271100）.