ADAM28反义核酸对人牙周膜干细胞增殖、分化和凋亡的影响

Effects of ADAM28 AS-ODN on proliferation, differentiation and apoptosis of HPDLSC

目的:探讨金属蛋白酶解离素28(ADAM28)反义核酸(AS-ODN)对人牙周膜干细胞(HPDLSC)增殖、分化、凋亡特性的影响和可能的作用机制。方法:体外分离培养、鉴定HPDLSC,将设计合成的ADAM28反义核酸和正义对照(S-ODN)分别转染HPDLSC,应用四唑盐(MTT)比色法、酶动力学法和流式细胞术(FCM)检测ADAM28反义核酸对HPDLSC生物学特性的影响。采用SPSS13.0软件包中的SNK检验进行统计学分析。结果:ADAM28 AS-ODN组HPDLSC的增殖活性、增殖指数显著低于S-ODN组和未转染组,碱性磷酸酶(ALP)分泌水平及凋亡细胞百分比明显上升,差异显著(P<0.01)。结论:ADAM28 AS-ODN可显著抑制HPDLSC的增殖并影响细胞周期的变化,促进ALP的分泌活性,显著诱导HPDLSC的凋亡。

PURPOSE： To investigate the effects of a disintegrin and metalloproteinase 28 （ADAM28） antisense oligodeoxynucleotide （AS-ODN） on proliferation, differentiation and apoptosis of human periodontal ligament stem cell （HPDLSC） and possible mechanism. METHODS： HPDLSC was isolated, cultured in vitro and identified. Synthetic ADAM28 AS-ODN and S-ODN were transfected into HPDLSC, respectively. MTT ehromatometry, enzyme dynamics and flow cytometry （FCM） were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HPDLSC. Statistical significance was assessed by the Student-Newman-Keuls （SNK） test with SPSS 13.0 software package. RESULTS： In ADAM28 AS-ODN group, the proliferation activity and index of HPDLSC were significantly lower than those of S-ODN group and untransfected group, alkaline phosphatase （ALP） secretion level and percentage of apoptotic ceils went up obviously. Significant difference was detected between AS-ODN group and other groups （P〈0.01）. CONCLUSIONS： ADAM28 AS-ODN could inhibit HPDLSC proliferation and influence the changes of cell cycle, promote ALP secretion and induce HPDLSC apoptosis significantly.