银杏提取物对衰老小鼠学习记忆能力及海马BDNF表达的影响

Effects of Extract from Ginkgo Biloba on Learning, Memory and Expression of Hippocampal Brain-derived Neurotrophic Factor in Senile Mice Induced by D-galactose

目的探讨银杏提取物(EGb)对D-半乳糖致衰老小鼠学习记忆能力及海马区脑源性神经营养因子(BDNF)表达的影响。方法用D-半乳糖皮下注射制备衰老小鼠模型,同时腹腔注射不同剂量的EGb治疗。六周后,用开阔行为试验检测小鼠在新异环境中的自发行为,用westernblot检测海马BDNF蛋白表达水平。结果50mg/kg和100mg/kgEGb均可显著增加D-半乳糖致衰老小鼠在新异环境中的自发活动和探究行为(P<0.05),抵御D-半乳糖对小鼠大脑学习记忆功能损伤作用(P<0.05),并可显著提高衰老小鼠海马组织中BDNF的表达(P<0.05)。结论EGb可显著提高D-半乳糖所致衰老小鼠的学习记忆能力,并提示这种提高可能与上调其海马BDNF的表达有关。

Objective To explore the effect of the extract from ginkgo biloba （EGb） on learning, memory and the expression of hippocampal brain-derived neurotrophic factor（BDNF） in the senile mice induced by D-galactose. Methods Thirty-two mice were randomly divided into 4 groups of 8 mice each, i.e. blank control group, model group, low dose of EG （50 mg/kg） treatment group and high dose of EGb （100 mg/kg） treatment group. The senile mice model in the model and treatment groups induced by D-galactose. The activity of the mice was determined by the open field test in all the groups, where the ability of learning and memory of mice were examined by step down test. The expression of BDNF in the hippocampi was determined by western blotting in all the groups. Results The open field test showed that the activity of the mice in both the treatment groups significantly increased compared to that in the model group （P〈0.05）. The step down test showed that the latent period of step-down was significantly longer and the latent period of escape was significantly shorter in both the treatment groups than that in the model group （P〈O.05）. The expression of hippocampal BDNF was significantly enhanced in both the treatment group compared to that in the model group. Conclusions The learning, memory and open field activity in the senile mice induced by D-galactose may be improved by EGb. It is suggested that the improvement of learning, memory and open field activity may be related to the increase in the expression of hippocampal BDNF in the senile mice induced by D-galactose.