提高微囊化新生猪胰岛细胞移植生物相容性和疗效研究

Research on improving the biocompatibility and xenotransplantation effect of microencapsulated new born porcine islets

目的探讨微囊化新生猪胰岛细胞和睾丸Sertoli细胞联合移植以及改变微囊物理特性是否可以提高生物相容性和移植效果。方法动物分6组：实验组1、2，对照组1、2、3、4每组移植（2000±100）个微囊化新生猪胰岛样细胞簇和睾丸Sertoli细胞，每组移植受体7只，将各组微囊移植入受体腹腔内，观察微囊的生物相容性和移植物的生物活性。结果实验组1血糖值于移植后2周降至正常（187±27）mg／dL，明显早于其他各组。并维持至移植后6周。实验组1中腹腔附着微囊数少，其活性率在68％±11％，明显高于游离微囊组的48％±10％，P〈0．05。实验组1中微囊周围细胞过度增生反应最轻，实验组2中呈中度细胞过度增生。结论微囊光滑，规则，直径不宜过大（350±50）μm，包裹1～2个细胞团更有利于减轻囊周过度增生，睾丸Sertoli细胞通过某些因子抑制巨噬细胞的趋化作用，从而减轻细胞过度增生主要是炎性细胞浸润，进而提高移植效果。

Objective To investigate if cotransplantation of neonatal porcine islets and sertoli cells and modification of the capsules would improve the biocompatibility of the capsules and transplantation effect of the grafts. Methods （2000 ± 100） encapsulated new born porcine islets and Sertoli cells were transplanted respectively or together. Each group had 7 recipients. The biocompatibility of the capsules and bioactivity of the islets was observed after the grafts were implanted into the abdominal cavity of the recipients. Results In EG1, blood glucose recovered to normal level （187 ± 27）mg/dL at 2 weeks after transplantation. It was much earlier than that of other groups. The normal glucose could be maintained for 6 weeks after transplantation. Although the adhesive capsules in EG1 were fewer than those of other groups, AO-PI staining proved that the survival rate of adhesive capsules was （68% ± 11% ）, much higher than （48% ± 10% ） of free capsules in EG1 （P 〈0.05）. Pericapuslar overgrowth in EG1 was slight, medium in EG2. Conclusion The smooth, regular capsules in （350 ± 50） μm diameter, and containing 1 or 2 cell clusters are advantage to reduce pericapsular overgrowth. Sertoli cells could decreasethe perieapsular overgrowth by inhibiting the chemotaxis of macrophages and improve the transplantation effect.