Skp2蛋白和PTEN蛋白在子宫内膜疾病中的表达及相关性研究

Expression of Skp2 protein and PTEN protein in endometrial cancer and their relationship

目的探讨Skp2和PTEN在子宫内膜组织中的表达及其在子宫内膜癌发生、发展过程中的作用及两者的关系。方法采用免疫组织化SP法检测42例子宫内膜癌、35例子宫内膜不典型增生,23例复杂性增生及15例正常内膜中Skp2蛋白和PTEN蛋白的表达情况,并分析两者的相关性。结果子宫内膜癌中Skp2蛋白表达水平显著高于正常子宫内膜、复杂增生及不典型增生的子宫内膜,差异有显著性(P<0.05);PTEN蛋白的表达完全相反,且二者的表达呈负相关。结论Skp2蛋白和PTEN蛋白在子宫内膜癌的发生、发展中发挥重要作用,Skp2和PTEN的联合检测有助于判断子宫内膜癌的恶性程度和预后。

Objective To investigate the expression of Skp2 and PTEN in endometrial tissues and their correlation and the carcinogenesis progression. Methods The expression of Skp2 and PTEN protein was detected by immunohistochemieal methods in 42 cases of endometrial cancer, 35cases of endometrial dysplasia and 23 cases of endometrial hyperplasia complex and 15 cases of normal endometrium. The relation of Skp2 and PTEN protein was analyzed. Results The positive rate of Skp2 protein expression in endometrial cancer was significantly higher than that in endometrial dysplasia and endometrial hyperplasia complex and normal endometrium （P 〈 0.05）. While the positive rate of PTEN was opposite to that of Skp2,the expression of PTEN was significantly lower than that in non-cancerous tissues （P 〈 0.05 ） In endometrial cancer,there was negative correlation between expression of Skp2 and PTEN. Conclusion Skp2 protein and PTEN protein play important roles in origination and development of endometrial cancer and the combination detection of Skp2 protein and PTEN protein expression may be helpful in predicting the malignant degree and prognosis of endometrial cancer.