耐甲氧西林金黄色葡萄球菌的检测及耐药性分析

Analysis of methicillin-resistant Staphylococcus aureus to commonly used antibiotics

目的分析耐甲氧西林金黄色葡萄球菌的临床感染现状及耐药性。方法采用美国MicroScan全自动细菌分析仪鉴定细菌种类,并用纸片扩散法进行药敏试验。结果2006-2007年共分离出金黄色葡萄球菌270株,其中,耐甲氧西林金黄色葡萄球菌（MRSA）174株,检出率为64.4%。所有的分离株对万古霉素都敏感;MRSA对红霉素、克林霉素、庆大霉素、复方磺胺、利福平和左旋氧氟沙星的耐药率分别为94.8%、71.8%、67.8%、85.1%、15.5%和48.9%。结论MRSA的分离率显著增高,耐药性严重,临床细菌室应高度重视MRSA的检测。

Objective To analyze the incidence and resistance of methicillin-resistant Staphylococcus aureus（ MRSA ）. Methods The bacterial strains were automatically identified with MieroScan-Walk- Away-40, and then the antibiotic resistances were tested by standard Kirby-Bauer method. Results A total of 270 strains were isolated during 2006 - 2007, in which 174 were MRSA （ 64. 4% ）. All isolates were susceptible to vancomycin. The resistant rate of the isolates of MRSA to erythromycin,clindamycin, gentamicin, sulfamethoxazole, rifampin and levofloxaxin were 94. 8% , 71.8% , 67.8% , 85.1% , 15.5% and 48.9%. Conclusions The isolation rate of MRSA increased significantly. For effective supervision and control of Staphylococcus aureus infection, MRSA surveying should be included in clinical microbiology surveillence.