胰腺癌CXCR-4、MMP-2、VEGF的表达及其与肿瘤生物学行为的关系

Relationship between expressions of CXCR4, MMP-2 and VGEF and biological behavior of pancreatic cancer

目的探讨CXCR-4、MMP-2、VEGF在胰腺癌中的表达及其与胰腺癌生物学行为的关系。方法采用免疫组化PV6000法检测47例胰腺癌组织中CXCR4、MMP-2及VEGF的表达，分析它们与肿瘤病理参数的关系以及3种蛋白表达之间的关系。结果47例胰腺癌组织中CXCR-4、MMP-2及VEGF的阳性表达率分别为72．3％、66．0％和61．7％，三者均与胰腺癌的转移、临床分期和预后有关（X^2=5．84—12．69，P〈0．05），而与患者性别、年龄、肿瘤大小和组织学分化无关（x^2=0．03～4．27，P〉0．05）。胰腺癌组织中CXCR-4的表达与MMP-2和VEGF的表达均呈正相关（r=0．587、0．521，P〈0．01）。结论CXCR-4通过上调MMP-2及VEGF的表达，共同参与胰腺癌的浸润和转移。

Objective To investigate the expressions of CXCR4, MMP-2 and VEGF in pancreatic carcinoma and the relationship with biological behavior of pancreatic carcinoma. Methods Immunohistochemical PV6000 technique was used to detect the expression of CXCR-4, MMP-2 and VEGF in 47 cases of pancreatic carcinoma tissues, and the relationship between the expressions and pathologic parameters, and the relationship among the expressions of the 3 proteins were analysed. Results The expression rates of CXCR-4, MMP-2 and VEGF were 72.3%, 66.0% and 61.7% in pancreatic carcinoma tissues, and all these proteins were correlated with metastasis, clinical staging and prognosis （ X^2 = 5.84 - 12.69; P 〈0.05）. The expression of CXCR-4, MMP-2 and VEGF was not correlated with sex, age, tumor size and differentiation （ X^2 = 0.03 - 4.27 ; P 〉 0.05 ）. There was a positive relationship between the expression of CXCR-4 and MMP-2 or VEGF in pancreatic carcinoma （r = 0. 587, 0. 521 ; P 〈 0. 01 ）. Conclusions CXCR-4 could up-regulate the expression of MMP-2 and VEGF in pancreatic carcinoma, and they may contribute to metastasis and invasion of pancreatic carcinoma.