大鼠牙移动过程中龈沟液中基质金属蛋白酶的表达

Expression of matrix metalloproteinases in the gingival crevicular fluid of rats during tooth movement

目的观察大鼠牙移动过程中龈沟液中MMP-1和MMP-9含量的变化。方法在20只6周龄雌性Wistar大鼠上颌一侧安装正畸加力装置,另一侧作为对照侧。每周提取大鼠实验牙及对照牙的龈沟液,并应用SDS-PAGE,Western blot和Gel-Doc凝胶成像系统定量检测龈沟液中基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)与基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)含量的改变。采用SPSS11.0统计学软件,对实验各周龈沟液中MMP-1和MMP-9的含量进行方差分析。结果MMP-1和MMP-9含量在实验侧和对照侧均是自加力第1周开始升高(与0周相比P<0.05),第2周时含量大幅增多(与第1周相比P<0.001),至第3周时达高峰,从第4周开始回落,至第7周最低,但仍高于加力前的水平;实验侧MMP-1含量显著高于对照侧(P<0.05);实验侧与对照侧的MMP-9含量差异均无统计学意义(P>0.05)。结论龈沟液中MMP-1和MMP-9的含量在大鼠牙移动过程中先升高后降低,MMP-1较MMP-9与正畸牙移动关系可能更密切。

Objective To detect expression of MMPs in the gingival crevicular fluid of rats during tooth movement.Methods Orthodontic appliances were fixed onto the maxilla of 20 6-week-old female Wistar rats.The maxillary first molar in the experimental side was drawn medially by applying an excess force of 50 g,with both of the maxillary central incisors as anchorage.In the control side,the maxillary first molar was wound around the dental cervix with ligature wire.The gingival crevicular fluid of the rats was collected every week,and MMP-1 and MMP-9 were assayed in quantitation by using the biochemical techniques of SDS-PAGE,Western blot and the Gel Doc quantitative analysis system.Results The contents of MMP-1 and MMP-9 in the gingival crevicular fluid increased at the 1st week in both sides,and greatly increased at the 2nd week.It reached a peak at the 3rd week,and fell back from the 4th week.At the 7th week,the contents were the least but more than that before the appliances were fixed.There was statistical significance in the contents of MMP-1 between the experimental side and the control side at the respective weeks（P〈0.05）,and the experimental side was more than the control side.But no statistical significance was shown in the content of MMP-9 between the two sides（P〉0.05）.Conclusions MMP-1 and MMP-9 in the gingival crevicular fluid play some role in orthodontic tooth movement.MMP-1 may be more closely connected with tooth movement than MMP-9.