TLR4在膀胱尿路上皮癌中的表达及意义

Relativity of TLR4 expression in uroepithelium carcinoma of the bladder

目的探讨TLR4蛋白在膀胱尿路上皮癌(BUEC)中的表达及意义。方法膀胱BUEC组62例标本经病理复检,WHO和ISUP病理分级具低度恶性潜能的乳头状尿路上皮肿瘤(G1)5例,低度恶性乳头状尿路上皮肿瘤(G2)24例,高度恶性乳头状尿路上皮肿瘤(G3)33例。11例正常膀胱粘膜和13例癌旁膀胱粘膜组织标本作为对照组。应用免疫组化非生物素即用型二步法检测组织TLR4的表达。结果两对照组TLR4均100%呈强阳性表达,G1中60%强阳性、40%弱阳性,G2中21%弱阳性、79%阴性,G3中3%弱阳性、97%阴性,随肿瘤病理分级上升,TLR4阳性表达率逐渐下降。结论TLR4表达与BUEC分级相关,可成为估计肿瘤预后的一项指标。

Objective To study the relativity of TLR4 expression in uroepithelium carcinoma of the bladder（BUEC）.Methods Paraffin-embedded tissue sections from 62 patients with BUEC were!re-examined.Based on the pathological diagnosis standard of WHO and ISUP,5 cases were urothelial neoplasm of low malignant potential（G1）,24 cases were low grade papillary urothelial carcinoma（G2）and 33 cases were high grade papillary urothelial carcinoma（G3）.11 specimens obtained from normal bladders and 13 obtained from adjacent tissues of tumors served as the controls.Expression of TLR4 was detected by immunohistochemistry.Results All the controls strongly expressed TLR4,while only 60% of G1 strongly expressed TLR4,40% of G1,21% of G2 and 3% of G3 had low expressions of TLR4,and 79% of G2 and 97% of G3 almost did not express TLR4.With the progression of tumor grade,positive rate of TLR4 expression was decreased（P=0.000）.Conclusion In uroepithelium carcinoma of the bladder,TLR4 expression is significantly associated with tumor grade,and a decrease of TLR4 expression is a prognostic factor for predicting progression of BUEC.