IGF-1和生长抑素-14对甲状腺相关眼病患者眼眶成纤维细胞增殖的影响

Effects of IGF-1 and Somatostatin-14 on the Proliferation of Cultured Orbital Fibroblasts of Thyroid-associated Ophthalmopathy Patients

目的:探讨重组人IGF-1和生长抑素-14单独及联合应用对甲状腺相关眼病患者眼眶成纤维细胞增殖的影响。方法:体外培养甲状腺相关眼病患者眼眶成纤维细胞,采用四甲基偶氮唑盐(MTT)比色法检测不同浓度及作用时间的重组人IGF-1和生长抑素-14对细胞增殖的作用。结果:50μg/L以上浓度IGF-1对眼眶成纤维细胞有促增殖作用,5nmol/L以上浓度的SST对眼眶成纤维细胞的生长有抑制作用,均呈量效和时效关系。IGF-1和SST联合应用时,50nmol/L以上的SST能阻断100μg/L的IGF-1促细胞增殖的作用。结论:IGF-1可刺激眼眶成纤维细胞增殖。SST能抑制眼眶成纤维细胞增殖,并能阻断IGF-1促进细胞增殖的作用,此抑制作用与浓度有关,提示SST能直接通过受体后机制抑制IGF-1诱导的眼眶成纤维细胞增殖。

Objective： To evaluate the effects of recombinant human IGF-1 and/or somatostatin-14 on the proliferation of cultured orbital fibroblasts of thyroid-associated ophthalmopathy（TAO） patients. Methods： Cultured orbital fibroblasts from 4 severe TAO patients were exposed to IGF-1 and/or SST-14 at various concentrations for 1, 2, and 3d respectively. The proliferation of the cells was studied with MTT assay. Results： Compared with that of the control group, the proliferation of cultured orbital fibroblast was enhanced by IGF-1 at 50μg/L or higher concentrations, while it was inhibited by SST-14 at 5nmol/L or higher concentrations. These effects were in both dose- and time-dependant way. The promotive effect of IGF-1 at 100μg/L on cultured fibroblasts could be blocked by SST-14 at 50nmol/L or higher concentrations. Conclusion： IGF-1 can promote the proliferation of the cultured orbital fibroblast. SST-14 can inhibit the proliferation of the cells, and block the promotive effect of IGF-1. These findings suggest that SST-14 may decrease the proliferative effect of IGF 1 through postreceptor inhibition mechanism.