紫外线和过氧乙酸对腺病毒灭活效果的观察

The Inactivation Effect of Ultraviolet Light and Peracetic Acid on Adenovirus

目的:寻找对腺病毒灭活的有效方法,来预防大规模生产腺病毒时所致的生产细胞的污染。方法:采用紫外线照射和不同浓度过氧乙酸的灭活方法灭活腺病毒,并将处理后的病毒加入到正常人胚肾293细胞中培养,观察细胞是否出现病变作为灭活效果判断标准。结果:我们发现一定强度紫外线照射至少2 h以上才能完全灭活腺病毒,过氧乙酸达到1%的浓度和至少作用30 min,才能将完全病毒灭活。结论:紫外线照射2 h以上能有效灭活腺病毒,我们可以用于操作台的腺病毒灭活;1%浓度的过氧乙酸作用30 min以上能有效将腺病毒灭活,我们可以运用于实验室大空间的腺病毒灭活。

Objective： To find the way to inactivate the contaminated adenovirus in the air of clean room and laminar hood. Methods： We mix the virus after different treatment with human embryonic kidney cell line （293 cell） to observe whether cytopathic effect appeared during five-day-culture. Results： In this research we found that at least 2-hour exposure under ultraviolet light can kill the virus. Furthormore, mixture of adenovirus and 1 % peracetic acid for at least 30 minute can inactivate the virus efficiently. Conclusion： More than 2-hour exposure under ultraviolet light can kill the virus, we can use this in the console＇s inactivated. 1% peracetic acid for more thant 30 minute can inactivate the virus, for this way we can use it inactivate the entire laboratory＇s virus.