白介素4对乙型肝炎病毒体外复制和表达的影响

The effect of interleukin-4 on the replication and express of hepatitis B virus in vitro

目的探讨IL-4对HepG2．2．15细胞表达HBsAg和HBeAg及HBVDNA复制的抑制作用，认识IL-4在抗HBV感染中的作用。方法通过MTT比色法检测不同浓度重组IL-4（rIL-4）对HepG2．2．15细胞的细胞毒性作用，用ELISA检测rIL-4对HepG2．2．15细胞不同时相HBsAg和HBe心分泌的作用，用实时荧光定量PCR检测rIL-4对HBVDNA合成的影响。结果rIL-4的各浓度对HepG2．2．15细胞无细胞毒性作用；rIL-4处理HepG2．2．15细胞后，不同浓度组HBsAg、HBeAg及HBVDNA含量在96h的差异具有统计学意义（P〈0．05），但rIL-4的浓度低于100ng／mL的各组，HBsAg和HBVDNA含量在处理48h时，各组间无明显差异（P〉0．05）。此抑制作用随rIL-4浓度的增加而增强。结论rIL-4在体外有直接的抗HBV作用。

Objective To explore the iuhibitory effect of IL-4 on HBsAg, HBeAg and HBV DNA cultured in HepG2.2.15 cell line, and to further provide a theoretical evidence and an experimental basis for investigating the inhibitory effect of IL-4 on HBV infection. Methods The cytotoxic effects of different concentration of recombinant IL-4 （rIL-4） on HepG2.2.15 cells were detected by MTF assay. The inhibition to HBsAg and HBeAg were measured by ELISA. The quantity of HBV DNA was detected with real-time fluorescence quantitative PCR（FQ-PCR）. Results MTT showed that different concentration of rlL-4 had no cytotoxic effect on HepG2.2.15 cells..There were statistical differences on HBsAg, HBeAg and HBV DNA levels among different concentration groups at 96 h after rIL-4 treatment（ P 〈 0.05）. However, there were no differences on HBsAg and HBV DNA levels at 48 h in those groups of 25-100μg/mL rIL-4 concentration. The suppressive effect increased with increasing rIL-4 concentrations. Conclusions These results suggest that rIL-4 at non-cellulotoxic concentration can suppress HBsAg and HBeAg expression and decrease the HBV DNA replication in HepG2.2.15 cell line.