抗-HCV阳性血清HCVRNA检测与基因分型的研究

Detection and genotyping of HCV RNA in anti-HCV positive serum

目的检测抗．HCV阳性血清中的HCVRNA并进行HCV基因分型。方法采用荧光定量PCR法检测85例大连地区抗．HCV阳性患者血清中HCVRNA，应用型特异性引物逆转录套式PCR法对HCVRNA阳性样本进行基因分型。结果85例的抗-HCV阳性患者中，HCVRNA阳性65例（76．5％），其中基因分型1b型32例（49．2％），2a型29例（44．6％），未分型4例（6．2％）。结论抗-HCV阳性并非HCV直接标志，大连地区HCV基因1b型和2a型基本相等。

Objective To detect and do genotyping of HCV RNA in anti-HCV positive serum. Methods HCV RNA was detected by fluorescent quantitation PCR in anti-HCV positive serum of 85 cases in Dalian area, and genotype was detected by type specificity primer reverse transcription nest PCR in HCV RNA positive specimens. Results In 85 cases of anti-HCV positive specimens, there were 65 cases of HCV RNA positive （76.5%）. In the HCV RNA positive serum, there were 32 cases of lb genotype （49.2%）, 29 cases of2a genotype （44.6%）, 4 cases of the others （6.2%）. Conclusions Anti-HCV positive is not direct mark for hepatitis C diagnosis, quantity of lb genotype is nearly equal to 2a genotype of hepatitis C virus in Dalian area.