可溶性趋化因子对人单核细胞表达及基质金属蛋白酶-9的影响

Effect of Fractalkine on the Expression and Secretion of MMP-9 in Human Monocytes

目的观察趋化因子Fractalkine(CX3CL1,Fkn)对人单核细胞株U937细胞表达与分泌基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的影响。方法使用不同浓度的人重组Fkn干预U937细胞,RT-PCR法检测细胞MMP-9基因表达,明胶酶谱法检测细胞上清中MMP-9水平。结果不同浓度人重组Fkn干预U937细胞MMP-9基因表达及上清中MMP-9水平低于空白对照组。结论Fkn可抑制U937细胞表达与分泌MMP-9。

Objective To study the effect of Fractalkine （ CX3CL1, Fkn） on the expression and secretion of matrix metalloproteinase-9 （MMP-9） in cultured U937 cells （ human monocyte line）. Methods The cultured U937 cells were incubated with recombinant human Fkn for 24 h. The mRNA expression of MMP-9 was analyzed by RT-PCR. The production of MMP-9 in the supernatant was analyzed by gelatin zymography. Results The level of MMP-9 mRNA and production in the cells incubated with recombinant human Fkn decreased compared with control group. Conclusion Fkn can inhibit the expression and secretion of MMP-9 in cultured U937 cells.