灵芝多糖对失血性休克兔一氧化氮及肠黏膜损伤的影响

Effects of GLP on Intestinal Mucosa Injury and Changes of Nitric Oxide Content in Hemorrhagic Shock Rabbits

目的观察失血性休克再灌注时NO、NOS的变化与肠黏膜损伤的关系及灵芝多糖(GLP)的影响。方法复制家兔失血性休克再灌注模型,随机分成假手术组(S组)、生理盐水再灌注组(NS组)和质量分数为1%的GLP再灌注组(LS组),分别于放血前、休克40min、再灌注40min和90min时观察细菌移位情况及血浆NO含量;于再灌注90min时,检测肠黏膜NOS活性、NO的含量;同时取回肠末端观察肠黏膜的损伤情况。结果①随着再灌注时间的延续,NS组血液细菌阳性率增加,细菌移位增加,明显高于LS组和S组,再灌注90min时肠黏膜损伤也明显重于LS组和S组;②休克40min时,NS组和LS组血浆NO含量明显高于S组和休克前,NS组再灌注后血浆NO进一步升高,明显高于LS组,肠黏膜NO含量和NOS活性也明显高于S组和LS组。结论GLP可降低失血性休克再灌注过程中NOS活性、NO含量以及肠黏膜损伤。

Objective To observe the intestinal mucosa injury and changes of nitric oxide（NO） content in hemorrhagic shock/reperfusion（HS-R） rabbits and the effect of ganoderma lucidum polysaccharide （GLP） on them. Methods With the model of HS-R in rabbits, the animals were divided into three groups： sham operation group（ group S）, reperfusion with NS group（ group NS）, repeffusion with 1% GLP group（ group LS）. The incidence rates of bacterial translocation were observed at the time of pre-shock, shock 40 min, postreperfusion 40 min and 90 min respectively. NO content in serum and intestinal mucosa, the activity of NOS in intestinal mucosa, the degree of intestinal injury were examined at the time of post-resuscitation 90 min. Resuits ① With the extension of reperfusion time the positive rate of blood bacteria increased gradually in NS group, which was significantly higher than that of group S and group LS（P 〈0.05）, meanwhile the degree of intestinal injury in group NS was more severe than that of group S and group LS（P 〈0.05）. ② NO content in serum of group NS and group LS was increased significantly compared with that before shock and in group S（ P 〈 0. 05）. NO content in serum was further increased after reperfusion with NS, which was significantly higher than that in group LS. NO content and NOS activity in intestinal mucosa of group NS we＇re significantly higher than those in group LS and group S（P 〈 0.05）. Conclusion The intestinal mucosa injury in HS-R is related to the activation of NOS and the increase of No content, meanwhile GLP can reduce the intestinal mucosa injury in HS.