免疫磁性纳米颗粒热疗治疗艾滋病初探

Study on HIV hyperthermia by immune-magnetite nanoparticles

目的探讨将HIV多抗与磁性纳米微粒偶联制备免疫磁性纳米微粒,用于HIV的靶向性加热治疗,并对其在交变磁场下的升温能力进行检测。方法采用共沉淀法制备四氧化三铁纳米材料,选择氨基硅烷偶联剂对磁性纳米微粒进行修饰,通过一定浓度戊二醛活化将抗HIV多抗交联制备免疫磁性纳米微粒,并对其在交变磁场下的升温能力进行检测。结果磁性纳米颗粒经氨基硅烷修饰后平均粒径为30nm左右。戊二醛活化的磁性纳米微粒与抗HIV多抗具有较高的结合率,可在体外稳定保存,具有良好的交变磁场下升温能力。结论抗HIV多抗免疫磁性纳米微粒的结合率、稳定性和交变磁场下升温能力可满足进一步的HIV靶向性加热治疗。

[ Objective ] To study a method synthesized immune-supermagnetite nanoparticles with anti-HIV antibody and iron oxide nanoparticles as a new way for targeting hyperthermia of HIV. [ Methods ] The immune-supermagnetite nanoparticles were synthesized by precipitation method and modified by silicane coupled agent. Glutaraldehyde was used as an activator to couple anti-HIV antibody and iron oxide nanoparticles. The feasibility of hyperthermia using magnetite nanoparticle-loaded anti-HIV antibody was evaluated under an alternating magnetic field. [Results] The mean size of supermagnetite nanoparticles＇with silicane was 30 nm and the synthesized efficiency of nanoparticles and anti-HIV antibody was high. Moreover, the immune-supermagnetite nanoparticles were conserved stably at 4℃ and the temperature of nanoparticles rose as high as the demand for the clinical hyperthermia under an ahernating magnetic field. [ Conclusion ] These results suggest that this novel therapeutic tool can be applicable to the further targeted research of HIV hyperthermia.