重组组织型纤溶酶原激活剂预防大鼠腹腔粘连的实验研究

Experimental study on prevention of the peritoneal adhesion by recombinant tissue-type plasminogen activator on rats

目的探讨重组组织型纤溶酶原激活剂(rt-PA)对于腹腔粘连的预防价值。方法20只SD大鼠随机分为2组制作腹腔粘连模型,rt-PA组和生理盐水(NS)组分别给予rt-PA和生理盐水一次性腹腔灌注。术后21d分别处死动物,观察腹腔粘连程度并进行病理组织学检查。结果rt-PA组的腹腔粘连与NS组比较,具有非常显著性降低(P<0.01),此外,病理组织学检查结果也表明经rt-PA灌注后减轻了腹腔粘连。结论rt-PA能够有效预防肠粘连的发生。

[ Objective] To evaluate the value of recombinant tissue-type plasminogen activator （rt-PA） on prevention of peritoneal adhesion. [ Methods ] 40 SD rats were divided randomly into two groups to reconstruct peritoneal adhesion model, then the rats in rt-PA group and NS group were respectively received once intracavity perfusion with rt-PA and NS. After the operation 21 days later, the animals were scarified, and that the severity of peritoneal adhesion was observed and pathohistological changes were detected. [Results] The rats of peritoneal adhesion in rt-PA group demonstrated a significantly reduction in comparison of the ones in NS group （P 〈0.01）. Pathohistological detection also showed the reduction of peritoneal adhesion by rt-PA intracavity perfusion. [ Conclusion ] The rt-PA can efficiently prevent the peritoneal adhesion.