复智散对阿尔茨海默病大鼠海马内源性神经干细胞增殖的影响

Effect of Fuzhisan(FZS) on Endogenous Stem Cells of the Hippocampus in Alzheimer Disease Model Rats

目的观察复智散(FZS)对Alzheimer病(AD)模型大鼠海马齿状回内源性神经干细胞增殖的影响。方法采用β淀粉样蛋白25-35(Aβ25-35)侧脑室注射制作AD大鼠模型。采用Morris水迷宫检测大鼠的学习记忆能力,免疫荧光检测大鼠海马5-溴脱氧尿嘧啶(BrdU)阳性细胞表达,免疫组化检测海马增殖细胞核抗原(PCNA)阳性细胞表达,并对海马齿状回下颗粒层、海马门、分子层分别进行BrdU、PCNA阳性细胞计数。结果与模型组比较,健康对照组、假手术组和FZS治疗组大鼠水迷宫实验中的平均逃避潜伏期缩短(P<0.05),齿状回颗粒细胞下层BrdU、PCNA阳性细胞数量明显增加(P<0.05),但后三组之间比较差异无统计学意义。结论 FZS可促进AD模型大鼠海马齿状回内源性神经干细胞增殖。

Objective To investigate the effect of Fuzhisan （FZS） on endogenous neurogenesis in the dentate gyrus（DG） of the hippocampus in Alzheimer disease （AD） model rats. Methods The AD model rats were induced by intracerebroventricular microinjection of Aβ25-35, Fzs was intragastric administration 30 days after the microinjection for four weeks. The learning and memory ability of rats was detected by the water maze test. S phase cells were labeled with BrdU. The BrdU, PCNA positive expression in DG was detected by immunoflourescence and immunohistochemistry method respectively. The total number of BrdU, PCNA positive cells in the subgranular cell layer, hilus and molecular layer of DG was recorded. Results Compared with the AD model group, the rats in the normal control group, sham operated group and FZS treatment group not only shorten the average escape latency in the water maze （P〈0. 05）, but also increased the number of BrdU and PCNA positive cells in subgranular cell layer （P〈0. 05）, there was no significant difference of the number of BrdU and PCNA positive cell among the later three group. Conclusions The FZS compounds treatment may promote the cell proliferation in the DG of the hippocampus of the AD model rats.