瑞昌山药组培快繁研究

Research on the Tissue Culture and Rapid Propagation of Ruichang Yam

[目的]探讨瑞昌山药组培快繁的技术环节。[方法]以瑞昌山药茎段为外植体,通过组织培养试验研究了不同激素及其浓度对愈伤组织形成、丛生芽诱导和生根的影响。[结果]70%乙醇和0.1%HgCl2结合对外植体的的消毒效果最佳。培养基MS+6-BA2.0mg/L+NAA0.2mg/L中的腋芽生长最好,培养基MS+6-BA1.0mg/L+NAA0.2mg/L、MS+6-BA1.0mg/L+NAA0.5mg/L、MS+6-BA2.0mg/L+NAA0.2mg/L和MS+6-BA2.0mg/L+NAA0.5mg/L中腋芽的诱导率分别为52%、43%、84%和48%。在继代培养中,采用原诱导培养基诱导丛生芽的效果较差,降低6-BA的浓度有利于丛生芽的诱导。培养基1/2MS+IBA1.5mg/L+NAA0.3mg/L+活性炭0.2mg/L中幼苗的生根效果较好。[结论]该研究为组织培养技术在种苗繁育中的应用奠定了基础。

[ Objective ] The aim of the research was to discuss the technical links in the tissue culture and rapid propagation of Ruichang yam. [ Method] With the stem segments of Ruiehang yam as cxplants, the effects of different hormones and their conch, on callus formation, tuftedbud induction and rooting were researched through tissue culture experiments. [ Result] The disinfection efficacy of combing 70% alcohol with 0.1% HgCI2 on the explants was best. The axillary buds grew best in the medium MS ＋6-BA 2.0 mg/L ＋ NAA 0.2 mg/L, the induction rates of axillary buds in the media MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.2 rag/L, MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.5 rag/L, MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.2 mg/L and MS ＋6-BA 2.0 mg/L ＋ NAA 0.5 mg/L were 52% ,43%, 84% and 48% resp. In subculture, the efficacy of inducing tufted- bud with the original induction medium was worse and reducing the conch, of 6-BA was helpful to tufted-bud induction. The rooting efficacy of seedlings was better in the medium 1/2 MS ＋ [BA 1.5 mg/L ＋ NAA 0.3 mg/L ＋ active carbon 0. ：2 mg/L. [ Conclusion] Tbc research lald a basis for the application of tissue culture technology in seedling breeding. Key words Ruichang yam; Tissue culture; Callus ; Tufted-bud induction