不同发酵条件对绿粘帚霉(RCEF4099)抗辣椒灰霉活性的影响

Effect of different fermentation conditions on anti-Botuytis cinerea activity by Gliocladium virens L. RCEF4099

[目的]为获得初步优化的发酵工艺条件及提高绿粘帚霉的发酵产量提供依据。[方法]通过单因子和正交试验研究了一系列主要培养条件(碳源、氮源、营养性因素、非营养性因素)对该菌株抗菌活性的影响。[结果]菌株RCEF4099最佳液体培养基配方为:40%葡萄糖、0.5%白砂糖、1.5%干酵母、0.02%K2HPO4;最佳培养条件为:装液量40/100ml(V/V),接种量10%(V/V),培养时间为5d,摇床转速160r/min。[结论]获得了初步优化的发酵工艺条件。

[ Objective ] The study was to obtain preliminary optimization of fermentation conditions and provide the reference for the improvement of fermentation yield of Gliocladium virens. [ Method] Single factor and orthogonal experiments were used to study the effect of a series of culture conditions such as carbon, nitrogen, nutrient factors, non-nutritional factors to the antimicrobial activity of this strains. [ Result ] The optimum medium compinents were： 4% of glucose, 0.5% of commercial sugar, 1.5% of dry yeast, 0.02% of K2HPO4 ; the optimum culture conditions were： aeration rate was 40/100 ml （V/V）, inoculation level was 10% （V/V） , fermentation period was 5 d, rotation was at 160 r/min. [ Conclusion] The preliminary optimization of fermentation conditions were obtained.