超干燥贮藏对蓝桉种子发芽及生理生化特性的影响

Effects of Ultra-dry Storage on Germination and Physiological and Biochemical Characteristics of Eucalyptus globulus Seeds

[目的]为蓝桉种子的常温贮藏提供理论依据。[方法]以干燥处理后含水率分别为9%、8%、7%、6%、5%、4%、3%、2%、1%并在常温条件下密封贮藏300、540d及未经干燥处理分别在常温和低温条件下贮藏300、540d(对照)的蓝桉种子为材料,测定不同处理种子的发芽、幼苗生长及生理生化指标。[结果]超干燥处理的蓝桉种子(含水率9%～1%)常温密封贮藏300、540d后,种子发芽率、发芽势、细胞膜透性、脱氢酶活性、α-淀粉酶活性总体高于常温对照组而接近低温对照组,部分指标甚至高于低温对照组;当种子含水率为2%时,种子的发芽能力、幼苗生长及生理生化特性最好。[结论]蓝桉种子超干燥处理后长期常温贮藏是完全可行的,且种子最佳含水率为2%。

[ Objective ] The study was to provide the theoretical basis for room temperature storage of Eucalyptus globulus seeds. [ Method ] With E. globulus seeds sealed and stored for 300, 540 days at room temperature after drying treatment with moisture content of 9%, 8% , 7% , 6% , 5% , 4% , 3% , 2% , 1% and E. globulus seeds sealed and stored for 300, 540 days at room temperature and low temperature （CK） as the materials, their seed germination, seedling growth and physiological and biochemical indexes in different treatments were detected. [ Result] The seed germination rate, germination energy, cell membrane permeability, activities of dehydrogenase and α-amylase of E. globulus seeds sealed and stored for 300, 540 days at room temperature after ultra-dry treatment （with moisture content of 9% - 1% ） were generally higher than those of E. globulus seeds in room temperature CK group and close to those of E. globulus seeds in low temperature CK group, and some indexes even higher than those of E. globulus seeds in low temperature CK group. The germination capacity, seedling growth and physiological and biochemical characteristics of E. globulus seeds were the best when their moisture content was 2%. [ Conclusion ] It was completely feasible for long-term storage of E. globulus seeds at room temperature after ultra-dry treatment, and the optimal moisture content of the seeds was 2%.