摘要
采用分段取样，测OD260，及电镜观察等方法，对用等密度梯度离心法提纯的传染性支气管炎病毒粒子在蔗糖溶液中的病毒富集带和纤突丢失程度进行了分析和总结。提出梯度离心后30％～50％区带蔗糖溶液为较理想的取样位置。
The concentrated position and the losing of spike of infectious bronchitis virus particles which were purified by isopycnic centrifugations on sucrose gradients were analysed by reading at wavelengths of 260 urn and observing under electron microscope,the band of 30%-- 50% in sucrose gradients was thought as the ideal position for separating virus after isopycnic centifugation.
出处
《上海农业学报》
CSCD
1998年第3期93-95,共3页
Acta Agriculturae Shanghai
基金
上海市农业科学院青年科技基金
关键词
传染性
支气管炎病毒
等密度梯度离心
电镜观察
Isopycnic centrifugation
Electron microscopy
Infectious bronchitis virus
