支原体、衣原体感染导致儿童慢性咳嗽免疫功能紊乱的研究

The Research of the Immune Dysfunction in the Chronic Cough Children with the Mycoplasma and Chlamydia Infection

目的:研究肺炎支原体(MP)、肺炎衣原体(CP)感染对慢性咳嗽患儿免疫功能的影响,研究其引起慢性咳嗽的可能机制。方法:检测确诊为慢性咳嗽患儿正规治疗前后IL-2、TNF-α、和T淋巴细胞亚群变化,用t检验方法与健康对照儿童进行组间比较。结果:慢性咳嗽患者治疗前CD8+较对照组降低,两组差异显著(P<0.05);CD4/CD8较对照组结果有所增高,组间比较差异有意义(P<0.01)。治疗后CD4/CD8明显降低,组间比较差异有意义(P<0.01)。慢性咳嗽患儿治疗前IL-2、TNF-α水平均显著高于正常对照组(P<0.01)和治疗后(P<0.01),而治疗后IL-2与正常组无统计学差异(P>0.05),TNF-α在治疗后较对照组仍增高(P<0.05)。结论:MP、CP可以诱导慢性咳嗽患者体内T淋巴细胞亚群比例失调,并且引起TNF-α、IL-2等炎性因子分泌增加。

Objective：To investigate the change of immunc function in the chronic congh caused by infection of Mycoplasma and Chlamycha pnenmoniac in children.Methods：The children with chronic cough caused by infection of MP or CP and the children with no obronic cough were cnrolled in the study.The expression of IL-2 and TNF-α were detected by ELISA,And the flow cytmmetry（FCM） was ased to detect the chang of subgroup of T lymphocyle.The childnen with chronic cough were treated with Authromycin for two or four weeks .Results：in the chronic cough group,the level of CD8＋ decreased when compared with the control group（P〈0.01）,and the CD4/CD8 is higher than the control group（P〈0.01）,After treatment the CD4/CD8 decreased（P〈0.01） ,The expression of IL-2 and TNF-α incrcased in the chronic cough group（P〈0.01）,After treatment the expression of IL-2 was not significant different from the control group,while the TNF-α still increased.Conclusions：The children with chrouic cough caused by infection of MP of CP have the disproportion of immune function.