两种ELISA法与免疫酶法检测EB病毒的比较分析

Evaluate the Two ELISA Methods and the EIA Method in Detecting Epstenin-Barr virus

目的:比较分析两种ELISA法与免疫酶法(IE法)检测VCA-IgA,并作方法学评价。方法:两种ELISA法和免疫酶法检测36例鼻咽癌(NPC)病人及58例正常人的VCA-IgA。结果:欧蒙(EBV-ELISA)的灵敏度为97.22%、特异性为84.48%、诊断效率为89.36%,国产(EBVELISA)的灵敏度为38 89%、特异性为100%、诊断效率为76.60%,IE法灵敏度为100%、特异性为74.14%、诊断效率为84.04%。结论:好的ELISA法较优于IE法,可作为筛查和早期临床诊断NPC的首选方法。

Objective：To evaluate the two ELISA methods and the EIA method in detecting Epstein-Barr Virus.Methods：The seara of EVB-VCA-IgA in 36 Nasopharyngeal patients and 58 healthy adults were detected Using two ELISA methods and EIA method.Results：For the ELISA method of EUROIMMUN,the sensitivity is 97.22%,the specificity is 74.44%,the percent of detection is 89.36%,For the ELISA method of Interior the sensitivity is 38.89%,the specificity is 100%,the percent of detection is 76.60%,For the EIA menthod,the sensitivity is 100%,the specificity is 74.14%,the percent of detection is 84.04% respectively.Conclusions：In scrcening of Nasopharyngeal and clinical diagnose Nasopharyngeal of early,the better ELISA menthod may use for the first one.