摘要
目的:研究水芹醋酸乙酯提取物(Sf-Et)对大鼠脑缺血-再灌注损伤的保护作用,并探讨其机制。方法:取大鼠50只,随机分为5组,即假手术组、脑缺血-再灌注模型组、Sf-Et(200,100mg.kg-1)剂量组、维拉帕米(Ver)(0.2mg.kg-1)、阳性组,每组10只。采用阻断大鼠双侧锁骨下动脉及颈总动脉的方法,制备大鼠脑缺血30min-再灌注60min损伤模型,观察Sf-Et对模型大鼠脑缺血前、缺血30min、再灌注60min后的脑电图(EEG)、血清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、胞浆内钙离子(Ca2+)浓度及脑组织病理变化的影响。结果:Sf-Et各剂量组均可促进缺血后EEG波幅的恢复;提高血清中SOD活性;降低MDA含量及胞浆内Ca2+浓度;减轻脑组织的病理变化。结论:Sf-Et对脑缺血-再灌注损伤有一定保护作用,其机制可能与其抗自由基,抑制钙超载有关。
OBJECTIVE To research the ethyl acetate extract from OenantheAquatic （Sf-Et） for protective effect of brain is chemia reperfusion injury in rats,and its mechanism. METHODS The 50 rats were divided into five groups randomly, sham group, ischemia-reperfusion model group, Sf-Et（200, 100 mg·kg^-1 ） dose group and verapamil（Ver） （0. 2 mg·kg^-1） positive group, 10 in each group. Rats were treated by blocking bilateral subclavian artery and the neck artery, rat injury model of 30 min ischemias 60 min reperfusion was established. EEG of Sf-Et before brain ischemia, after ischemia 30 min and reperfusion 60 min was observed, influence of superoxide dismutase （SOD） activity in serum, contents of malondialdehyde （MDA）, concentration of brain intracellular calcium（Ca^2＋ ） and pathological changes of brain tissue was studied. RESULTS Sf-Et can promote recovery of EEG amplitude of brain ischemia, raise SOD activity in serum, lower MDA content and Ca^ 2＋ concentration of brain cells,Sf-Et dose group could reduce the pathological changes in brain tissue. CONCLUSION Sf-Et for brain ischemiareperfusion injury have protective effects, its mechanism may be related to the anti-free radicals and inhibit calcium overload.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2009年第22期1901-1904,共4页
Chinese Journal of Hospital Pharmacy
基金
吉林省科委课题(编号:1997第48号)
