榄钱两株附生真菌混合培养次级代谢产物研究

Studies on the Secondary Metabolites Produced by Mixed Cultures of Two Epiphytic Fungi Isolated from a Fruit of Mangrove Avicennia marina

通过混合培养2株附生于南海榄钱的真菌产生了2个抗菌次级代谢产物(A和B),其结构通过完整的波谱数据(主要是2D NMR数据)分别解析为新曲霉酸(A)和曲酸(B)。化合物B的结构进一步通过单晶X-射线衍射分析得到证实。当单独培养这2株真菌时未能得到化合物A。研究结果进一步表明,混合培养技术有助于从海洋微生物中发现更多独特的天然产物。

A mixed culture of two epiphytic fungi from a rotten fruit of mangrove Avicennia marina produced two antibacterial secondary metabolites （A and B ）. Their structures were elucidated as neoaspergillic acid （A） and kojic acid （B） by comprehensive spectral methods （mainly by 2D NMR spectral methods）. The structure of compound B was further characterized by single crystal X-Ray diffraction analysis. The crystal of compound B belongs to monoclinic system, space group is P21/n. The unit cell parameters are： a =0.378 33 （3） nm, b=1.837 58 （14） nm, c=0.848 89 （7） nm, α=90°, β= 96. 752 0 （10）°, T =90°, V=0. 586 06 （8） nm^3 and Z =4. Compound A was not obtained when either strains was cultured individually. The results showed that the application of mixed culture technique would be contribute to discover more novel natural products from marine microorganisms.