大黄蒽醌提取物对罗氏沼虾抗高温应激的影响

Effects of Anthraquinones Extracted from Rheum officinale Baill on Prevention of High Temperature of Macrobrachium rosenbergii

将罗氏沼虾随机分成5组,每组3个平行,每个平行约1 000尾,第1组为对照组,投喂基础日粮,另外4组为试验组,在基础日粮中分别添加w为0.05%、0.10%、0.20%、0.40%大黄蒽醌提取物。饲养8周后,对虾进行连续48 h 35℃高温应激,测定其生长、应激前后血淋巴葡萄糖、胆固醇、甘油三酯、溶菌酶、一氧化氮(NO)等的变化。结果表明:与对照组相比,添加w=0.10%的大黄蒽醌提取物显著提高了虾的增重率、降低了饵料系数,w=0.40%大黄蒽醌提取物显著增加了血淋巴溶菌酶含量。高温应激后,与对照组相比,添加w为0.1%和0.20%大黄蒽醌提取物仍保持较高血淋巴溶菌酶、一氧化氮浓度。高温应激试验表明:对照组死亡率达60%,添加w为0.10%和0.20%大黄蒽醌提取物小于30%。因此添加w为0.10%和0.20%大黄蒽醌提取物提高了机体抗应激能力,并对高温引起虾的死亡有一定的保护作用,促进了虾的生长。

In order to research the effects of anthraquinones extracte from Rheum officinale Baill on prevention of high temperature of Macrobrachium rosenbergii, Macrobrachium rosenbergii were divided into five groups randomly. The controls were fed with basal diet, the treated groups were fed with basal diet supplemented with 0.05%, 0. 10%, 0. 200%, 0. 40% anthraquinones extracte for 8 weeks, respectively. After that, shrimp were stressed by high temperature at 35 ℃ for 48 hours continuously. Then the growth and the changes of nitrogen oxide, lysozyme, glucose, triglyceride and cholesterol and in haemolymph before and after the stress were investigated. The results showed that 0.10% dose group significantly increased weight gains and reduced feed conversion rate, and 0.40% dose group significantly increased haemolymph lysozymes contents before the stress. After the stress, 0. 10% and 0. 20% dose groups were relatively higher in haemolymph lysozymes and nitrogen oxides contents than those of the control. Stress also caused mortality in all groups of which 0. 10% - 0. 20% dose groups were less than 30%, but the control was about 60%. It is concluded that ingestion of 0. 10% -0. 20% dose of anthra- quinones extracte could help prevent negative effects of stress and improve shrimp growth.