针刺对宫内缺血脑损伤大鼠行为学及脑组织病理形态的影响

Acupuncture effect on ethology and brain morphology of histopathology in intrauterine ischemic brain damage rats

目的观察针刺疗法对宫内缺血脑损伤大鼠学习记忆能力、运动能力及脑组织HE染色病理形态的影响。方法采用结扎孕鼠双侧子宫动脉,完全阻断血供25min娩出胎鼠,制成宫内缺血脑损伤模型,成功模型随机分入针刺组和模型组,每组各8只;正常对照组剖腹产取出胎鼠,8只;其余胎鼠丢弃。正常对照组和模型组不治疗,针刺组于出生后14日龄至30日龄行针刺治疗,每日针刺1次,不留针。所有实验鼠于14日龄及30日龄进行行为学测试(Y型迷宫、悬吊实验、斜坡实验),测试结束后断头取脑,HE染色观察各组脑组织病理形态的变化。结果(1)14日龄时各行为学指标比较,模型组和针刺组差异无统计学意义(P>0.05),该两组与正常对照组差异有统计学意义(P<0.05)。30日龄时,针刺组Y型迷宫错误反应次数、悬吊实验维持时间及得分、斜坡实验耗时等指标均介于正常对照组与模型组之间,与其余两组差异均有统计学意义(P<0.05)。(2)脑组织HE染色形态学观察结果表明,模型组出现了较严重的皮质和海马神经细胞丢失、变性,针刺组虽然较正常对照组仍有不同程度的神经细胞变性,但较模型组脑细胞形态和结构完整。结论针刺疗法对宫内缺血后大鼠学习记忆障碍、运动障碍有一定的改善,且对神经元形态和超微结构具有保护作用。

Objective To observe the acupuncture effect on intrauterine ischemic brain damage ratsr study memory ability,motor ability and HE staining pathological morphology of brain tissue. Methods To ligate bilateral uterine arteries of the pregnant rats and completely block the blood supply for 25min. Then birth fetus rats were made the intrauterine ischemic brain damage models. The successful-models were randomly divided into the acupuncture group and the model group, 8 rats each. The normal group came out of normo-caesarean,8 rats. Discard the remaining fetal rats. Results （1）Ethology index comparison：on 14d,the model group and the acupuncture group did not have the difference （P〉0.05） ,these two groups had the difference with the normal group （P〈 0.05）. On 30d,comparing the error number of maze Y（EN） ,the maintenance time and the score of grasping experiment, the time- consuming of Pitch experiment in the three groups, those indexes of acupuncture group were situated between the normal group and the model group with significance difference （P〈0. 05）. （2）The result of HE staining pathological morphology of brain tissue strawed that,the model group appeared more serious cortex and hippocampus nerve cell loss and degeneration. Although the acupuncture group still had varying degrees of nerve cell degeneration, its morphology and structural integrity of brain cells were better than those of the model group. Conclusion Acupuncture can improve the study memory ability,motor ability of intrauterine ischemic brain damage rats,also can protect the morphology and ultrastructure of the nerve cells.