多壁纳米碳管/二氧化硅复合膜中的联吡啶钌电化学发光法测定氧氟沙星的研究与应用

Determination of Ofloxacin Hydrochloride Based on Multiwall Carbon-nanotube/SiO_2 Sol-Gel-Ru(bpy)_3^(2+) Using Electrochemiluminescence

基于氧氟沙星对联吡啶钌(Ru(bpy)32+)电化学发光的增敏作用,建立了一种以多壁纳米碳管(MWCNTs)/二氧化硅-联吡啶钌复合物修饰的玻碳电极电化学发光检测氧氟沙星的新方法。利用溶胶-凝胶(sol-gel)固定化稳定的优点和纳米碳管的电催化作用,提高了传感器的电流响应。在最佳实验条件下,氧氟沙星浓度在4.0×10-6~1.0×10-4mol/L范围内与相对发光强度呈线性关系(r2=0.994 8),检出限(S/N=3)为2.0×10-6mol/L。连续平行测定2.4×10-5mol/L的氧氟沙星溶液5次,发光强度的RSD为1.8%。

Based on the sensitization effect of ofloxacin on Ru （bpy）3^2＋ electrochemiluminescence system, an electrochemiluminescence method for the determination of ofloxacin hydrochloride at multiwall carbon nanotube/SiO2 sol -gel -Ru （bpy）3^2＋ composite film modified glass-carbon electrode was described. A highly-sensitive sensor was prepared based on multi-walled carbon nanotubes modified electrode conbined with SiO2 sol - gel immobilization method. The fabrication procedure of the modified electrode, and experiment conditions and electrochemical characteristics were studied in detail. Under the optimum condition, the calibration curve showed a good linearity over otloxacin hydrochloride concentration in the range of 4.0×10^-6-1.0×10^-4mol/L（ r^2 = 0. 994 8 ） and the detection limit（S/N = 3 ） was 2.0×10^-6mol/L. The RSD for 5 times determination of 2.4×10^-5mol/L ofloxacin was 1.8% .