松嫩草原罗布麻各器官总黄酮和槲皮素的含量测定及药材抗氧化活性评价

Determination of Total Flavonoids and Quercetrin in Different Parts of Apocynum venetum from Songnen Grassland & Its Antioxidant Activity

目的:测定松嫩草原罗布麻各器官总黄酮和槲皮素含量并评价其体外抗氧化活性,为有效利用罗布麻资源提供参考。方法:以超声波辅助提取罗布麻中总黄酮,用比色法和高效液相色谱法分别测定罗布麻中总黄酮和槲皮素的含量,DPPH.和.OH清除法评价其抗氧化活性。结果:松嫩草原罗布麻花和叶中总黄酮、槲皮素含量较高,其次为茎;叶和花中总黄酮提取液抗氧化活性较强,茎次之。结论:松嫩草原罗布麻叶、花和茎均可以作为天然抗氧化剂原料。

OBJECTIVE： To determine the contents of total flavonoids and quereetin in different parts of Apocynum venetum collected in Songnen grassland and study their antioxidant activities so as to provide references for efficient utilization of the herbal plant. METHODS： Ultrasonic extraction was applied to extract total flavonoids. The content of total flavonoids was determined by chromatometry and the content of quercetin was determined by HPLC. The antioxidant activities of the extracts from different parts of A.venetum were evaluated by determining their abilities in scavenging 1, 1- diphenyl- 2 picrylhydrazyl （DPPH · ） radicals and· OH radicals. RESULTS： The contents of quercetin and total flavonoids were richer in both leaves and flowers of A. venetum than in stems. In addition, the total flavonoids extracted from the leaves and flowers of A.uenetum showed higher antioxidant activity than from the stems. CONCLUSION： The leaves, flowers and stems of A.venetum can all be used as raw materials of natural antioxidants.