摘要
目的:建立以高效液相色谱法测定痛经康口服液中原儿茶醛含量的方法。方法:色谱柱为Zorbax Eclipse XDB-C18(250mm×4.6mm,5μm),流动相为甲醇-水-甲酸(20∶79.5∶0.5),检测波长为280nm,流速为1.0mL.min-1,柱温为室温。结果:原儿茶醛进样量在0.025~0.5μg范围内与峰面积积分值呈良好的线性关系(r=0.999 9);平均回收率为101.1%,RSD=2.29%(n=6)。结论:本方法简单、准确,可用于痛经康口服液的质量控制。
OBJECTIVE: To develop an HPLC method for content determination of protocatechualdehyde in Tong- jingkang oral liquid. METHODS: Zorbax Eclipse XDB-C18(250 mm×4.6 mm, 5μm) was used as chromatographic column with mobile phase consisted of methanol-water- formic acid (20 : 79.5 : 0.5) at a flow rate of 1.0 mL · min^-1. The detection wavelength was set at 280 nm and the temperature of column was kept at room temperature. RESULTS: The linear range of protocatechualdehyde was 0.025--0.5 μg (r = 0.999 9) with an average recovery of 101.1% (RSD = 2.29%, n = 6) . CONCLUSION: The established method is simple, accurate and it is applicable for the quality control of Tongjingkang oral liquid.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第33期2599-2600,共2页
China Pharmacy
基金
广东中山市科技计划项目(20082A068)