摘要
采用不同的浓度乙醇提取苦瓜叶中的黄酮,以芦丁为标准品,采用NaNO2-Al(NO3)3比色法,测定提取液中的黄酮含量.结果表明,四种因数对提取率的影响分别是:乙醇浓度、料液比、提取时间、提取温度.乙醇提取法最优工艺为:乙醇浓度65%,提取时间4h,料液比1∶20,提取温度为80℃.
Adopting different consistency ethanol to extract flavone in balsampear leaf. Taking rutin as standard, adopting NANO2 -Al（NO3 ）3 colorimetry to determine the flavone contents in extraction liquid. The result shows that the four factors influence the extraction accordingly： ethanol consistency, liquid ratio, extraction time, extraction temperature. The best method of ethanol extraction technics： ethanol consistency 65 %, liquid ratio 1 ： 20, extraction time 4h, extraction temperature 80℃.
出处
《湘南学院学报》
2009年第5期64-66,共3页
Journal of Xiangnan University
基金
湖南省大学生研究性学习和创新性试验计划(〔2008〕No.271)
关键词
苦瓜叶
总黄酮
乙醇回流法
比色法
balsampear leaf
general flavone
ethanol circumfluence
colorimetry
