摘要
目的:观察葡萄糖对人单核细胞性白血病细胞株(human monocytic leukemia cell line,THP-1)巨噬细胞的载脂蛋白E(Apo E)表达的影响。方法:用佛波脂诱导THP-1细胞使其分化成巨噬细胞。再用含不同浓度葡萄糖的RPMI 1640培养基培养巨噬细胞72 h后,RT-PCR和Western Blot分别检测巨噬细胞Apo E的mRNA和蛋白的表达,同时用MTT法检测各组细胞活力。结果:葡萄糖呈剂量依赖性(20~30 mmol/L),明显抑制THP-1巨噬细胞Apo E的表达。结论:葡萄糖抑制THP-1巨噬细胞Apo E的表达,其可能是导致动脉粥样硬化的机制之一。
Objective:To observe the influence of glucose on the expression of Apolipoprotein E(Apo E) in human monocytic leukemia cell line ( THP-1 ) macrophages. Methods: THP-1 cells were induced to macrophages with phorbol, and then treated with RPMI 1640 culture medium containing diferent concentrations of glucose for 72 hours. Apo E mRNA and protein level were analyzed by RT^PCR and Western Blot respectively, meanwhile the viability of macrophages was assayed with MTT method. Results: Glucose significantly decreased Apo E expression of THP-1 macrophages in a dose-dependent manner( 20 -30 mmol/L). Conclusions :Glucose decreases Apo E expression of THP-1 macrophages ,which might be one of the mechanisms of proatherosclerosis of glucose.
出处
《蚌埠医学院学报》
CAS
2009年第11期953-955,共3页
Journal of Bengbu Medical College
基金
国家自然基金资助项目(30870954)
上海市科委自然科学基金资助项目(07ZR14071)