猪圆环病毒1型北京株全基因组克隆及序列分析

Cloning and Sequence Analysis of the Complete Genome of Porcine Circovirus Type 1 Beijing Strain

参照GenBank上猪圆环病毒1型（PCV1）全基因组序列设计1对引物，用PCR技术从北京一猪场的临床发病猪病变组织中扩增和克隆获得1株PCV1全基因组序列，并进行序列测定和分析。全基因组序列结果显示，该株PCV1基因组全长为1759bp，与国内外其他各株PCV1的核苷酸同源性为99．4％。主要开放阅读框（ORF）序列结果显示，该分离株与国内外其他PCV1毒株间的ORF1与ORF2的核苷酸同源性分别在97．5％～99．8％和92．7％～100％。虽然各个PCV1分离株之间的全基因组与主要ORF的核苷酸同源性均很高，但仍然呈现出一定的地域相关性。

A pair of primers for porcine circovirus type 1 （PCV1） was designed according to its sequences available in GenBanko the genome sequence of one PCV1 isolate was amplified by PCR assay with the tissues of the clinical diseased pig from a pig farm in Beijing. Then, the complete genome of the PCV1 isolate was cloned, sequenced and analyzed. The genome sequence results showed that this genome is 1 759 bp in size, and shared 99.4% identity of nueleotide with other PCV1 isolates selected from the GenBank. The sequences of the main open reading frame （ORF） revealed that there were about 97.5 %-99.8% and 92.7 %- 100 % identity of nucleotide in the ORF1 and ORF2 among the selected PCV1 isolates respectively. The present study indicated that there was a geographical correlation among the PCV1 isolates, although the identity of nucleotide in the genomes and main ORFs of the selected PCV1 isolates was relatively high.